Coding

Part:BBa_K1611000:Design

Designed by: Frederic Ros   Group: iGEM15_Evry   (2015-09-15)


IFNgamma


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 33


Design Notes

We performed a PCR site directed mutagenesis removing an enzyme restriction site in order to make it biobrick compatible. The mutation performed is silencious.


Source

This sequence was synthesised by IDT based on the genomic murine IFN gamma.

Organism: Homo sapiens (human)

References